A review of developments in the methodology and application of microemulsion electrokinetic chromatography.

MEEKC is a mode of CE, which utilizes microemulsion (ME) as the BGE to achieve separation of a diverse range of analytes. MEs are composed of nanometer-sized oil droplets suspended in aqueous buffer which are stabilized by the presence of a surfactant and co-surfactant. These MEs are commonly referred to as oil-in-water MEs and their application in MEEKC has been extensively examined. This review details advances in the theory, methodology, and application of MEEKC during the period 2010-2012. Areas covered include online sample concentration, advances in chiral separations, use of coated capillaries, chemometric approaches, and the use of novel additives to the ME system. This review also provides the reader with an introduction to MEEKC and a presentation of recent applications.

Recent developments in the methodology and application of MEEKC.

MEEKC is an electrodriven separation technique that utilises the unique properties of a microemulsion (ME) as a background electrolyte to achieve separation of a diverse range of solutes. MEs are composed of nanometre-sized oil droplets suspended in aqueous buffer, which is commonly referred to as oil-in-water ME. The droplets are stabilised by the presence of both a surfactant and co-surfactant. The use of water-in-oil MEs in MEEKC has also been investigated. This review details the advances in MEEKC-based separations from the period June 2008 - June 2010. Areas covered include online sample concentration, suppressed electroosmosis MEEKC, chiral separations, MEEKC-MS, MEEKC-ICP-MS and ME structure characterisation. The review also includes a fundamental introduction to MEEKC, along with a review of recent applications.

Recent advances in the methodology, optimisation and application of MEEKC.

MEEKC is an electrodriven separation technique. Oil-in-water microemulsions (MEs) and to a lesser extent water-in-oil MEs have been used in MEEKC as BGEs to achieve separation of a diverse range of solutes. The more common (oil-in-water) MEs are composed of nanometre-sized droplets of oil suspended in an aqueous buffer. Interfacial tension between the oil and aqueous phase is reduced close to zero by the presence of a surfactant and a co-surfactant. MEEKC is capable of providing fast and efficient separations for a wide range of acidic, basic and neutral, water-soluble and -insoluble compounds. This review details the advances in MEEKC-based separations from the period 2006 to 2008. Areas covered include online sample concentration, chiral separation, suppressed electroosmosis MEEKC, MEEKC-MS, and the use of MEEKC in predicting migration behaviour and solute characteristics. A fundamental introduction to MEEKC, along with the presentation and discussion of recent applications is also included.

Optimisation and validation of a rapid and efficient microemulsion liquid chromatographic (MELC) method for the determination of paracetamol (acetaminophen) content in a suppository formulation.

A rapid and efficient oil-in-water microemulsion liquid chromatographic method has been optimised and validated for the analysis of paracetamol in a suppository formulation. Excellent linearity, accuracy, precision and assay results were obtained. Lengthy sample pre-treatment/extraction procedures were eliminated due to the solubilising power of the microemulsion and rapid analysis times were achieved. The method was optimised to achieve rapid analysis time and relatively high peak efficiencies. A standard microemulsion composition of 33 g SDS, 66 g butan-1-ol, 8 g n-octane in 1l of 0.05% TFA modified with acetonitrile has been shown to be suitable for the rapid analysis of paracetamol in highly hydrophobic preparations under isocratic conditions. Validated assay results and overall analysis time of the optimised method was compared to British Pharmacopoeia reference methods. Sample preparation and analysis times for the MELC analysis of paracetamol in a suppository were extremely rapid compared to the reference method and similar assay results were achieved. A gradient MELC method using the same microemulsion has been optimised for the resolution of paracetamol and five of its related substances in approximately 7 min.

Recent advances in the development and application of microemulsion EKC.

Microemulsion EKC (MEEKC) is an electrodriven separation technique. Separations are typically achieved using oil-in-water microemulsions, which are composed of nanometre-sized oil droplets suspended in an aqueous buffer. The droplets are stabilised by a surfactant and a cosurfactant. The novel use of water-in-oil microemulsions has also been investigated. This review summarises the advances in the development of MEEKC separations and also the different areas of application including determination of log P values, pharmaceutical applications, chiral analysis, natural products and bioanalytical separations and the use of new methods such as multiplexed MEEKC and high speed MEEKC. Recent applications (2004-2006) are tabulated for each area with microemulsion composition details.